This book will serve as an ideal textbook for various introductory courses of Biotechnology offered in Life sciences (B.Sc. Biotechnology, B.Sc. Microbiology, B.Sc. Bioinformatics, M.Sc. Biotechnology, M. Sc. Microbiology, M.Sc. Botany, M.Sc. Zoology, etc.) and in agriculture (M.Sc. Genetics and Plant Breading, M.Sc. Plant Pathology, M.Sc. Entomology, etc.). This book can also be used as self-study material for obtaining basic knowledge and understanding of Biotechnology. This book contain Definition and History of Biotechnology, Genetic Engineering & Chimeric DNA, Cloning & Expression Vectors, Artificial Chromosomes, Methods of DNAPurification & Sequencing, Gene Isolation, Organo-chemical Gene Synthesis, DNASynthesis, Methods of Analysis of Gene Expression, Methods of Protein Sequencing & Protein Engineering, Microscopy, Centrifugation, Electrophoresis, Spectroscopy, Chromatography and Radioisotope Techniques.