Contents: 1. Applied Aquaculture Biofloc Technology 2. In-situ and Ex-situ Biofloc on Immune Response of Gift Tilapia 3. Fertilization Prototype on Biofloc Development in Gift Tilapia Culture 4. Distillary Spent Wash as a Carbon Source in Intensive Biofloc Nursery Rearing of Penaeus vannamei 5. In-vivo Study of Bacillus sp Isolated from Biofloc System in Gift Tilapia 6. Probiotic Potential of Bacillus Strains of Biofloc System on Growth Performance in Penaeus vannamei 7. Extra Cellular Polymer Substances (EPS) Producing Bacteria From Biofloc of Nile Tilapia Culture System using Distillery Spent Wash as Carbon Source 8 Ex-situ Production of Biofloc in In-Pond Raceways and Photobioreactor