The book covers from the very basic introduction on molecular
biology and diagnostics to the protocols of advanced assays that can
be offered by a diagnostic laboratory. There is detailed description on
the requirements for setting up a laboratory. The chapter on
laboratory mathematics will help the reader in preparation of
common laboratory solutions. The book contains basic protocols to
perform nucleic acid extraction from different sources. These
protocols can be effortlessly applied to isolate high quality DNA and RNA from human blood and tissues. The book
is profusely explained with various molecular diagnostic tools which are easy to understand and can be applied in
diagnosis of different cancers. This book also presents with examples of biomarker detection protocols for specific
cancer type using molecular diagnostic tools which can easily be reproduced. These protocols are very sensitive, cost
effective and can be performed to provide faster and accurate diagnosis.
The last part of the book deliberates on the quality assurance and quality control strategies of a molecular diagnostic
laboratory. Special emphasis is made on specific guidelines to design quality manuals for a molecular diagnostic laboratory.