Laboratory Techniques with Reagents and Solutions
U. D. Chavan
  • ISBN 13 : 9789351248439
  • year : 2018
  • language : English
  • binding : Hardbound
Contents: Preface. 1. Introduction. 2. Some basic concepts. 3. Types of stock standard solutions. 4. Equivalent concentration. 5. Buffer solutions. 6. pH. 7. (i). Spectrophotometry. (ii). Atomic absorption spectrophotometry. 8. Chromatography. 9. Enzyme assays. 10. Radioactivity and related calculations. References. Appendices. This book contains most suitable procedure of laboratory techniques. It describes the basic laboratory rules, equipment and instrumentation, basic concepts, types of stock standard solutions, equivalent concentration, normality, normal solution, molar solution, molal solution, percent solution, parts per million (ppm), various types of buffer solutions, pH, spectrophotometry, atomic absorption spectrophotometry, chromatography viz. paper chromatography, thin layer chromatography (TLC), gas chromatography (GC), liquid chromatography (LC), gas-liquid chromatography (GLC), high performance liquid chromatography (HPLC), polymerase chain reaction (PCR), scanning electron microscope (SEM), radiation, enzyme assays and radioactivity and related calculations. These equipment and instruments are commonly used in practical laboratory. Important references have been also cited for detailed reading. It is hoped that the book is quite useful for students, teachers, researchers, biochemists, chemist, food chemist and person engaged in various laboratories.